Violina Angelova

Agricultural University-Plovdiv, 12 Mendeleev street, Plovdiv, Bulgaria

2nd International Scientific Conference on Recent Advances in Information Technology, Tourism, Economics, Management and Agriculture – ITEMA 2018 – Graz, Austria, November 8, 2018, CONFERENCE PROCEEDINGS published by the Association of Economists and Managers of the Balkans, Belgrade, Serbia; ISBN 978-86-80194-13-4


A comparative research on the impact of organic meliorants on the uptake of heavy metals, micro and macroelements and phytoremediation potential of enhanced tobacco NBCu 10-8F3 has been carried out. The soil used in this experiment was sampled from the vicinity of the Non-Ferrous-Metal Works near Plovdiv, Bulgaria. The pot experiment was a randomized complete block design containing nine treatments and three replications (27 pots). The treatments consisted of a control (no organic meliorants) and compost and vermicompost meliorants (added at 5%, 10%, 15% and 30%, recalculated based on dry soil weight). Upon reaching commercial ripeness, the tobacco plants were gathered. Heavy metals, micro and macroelement contents in roots, stems and leaves of tobacco were analyzed by the method of the microwave mineralization. To determine the elements in the samples, inductively coupled emission spectrometry (Jobin Yvon Emission – JY 38 S, France) was used. The distribution of the heavy metals, micro and macroelements in the organs of the enhanced tobacco has a selective character and depended above all on the parts of the plants and the element that was examined. Pb, Zn, Cu, Fe, Mn, P and Mg distribution in tobacco decreases in the following order: roots > leaves> stems, and for Cd, K, and Ca – leaves >roots> stems. The high concentration of Cd in the leaves and the high translocation factor indicate the possibility of enhanced tobacco to be used in phytoextraction.

Tested organic amendments significantly influenced the uptake of heavy metals, micro and macroelements by the roots, stems and leaves of tobacco. A correlation was found between the quantity of the mobile forms and the uptake of Pb, Zn and Cd by the enhanced tobacco. The compost and vermicompost treatments significantly reduced heavy metals concentration in leaves and increased uptake of K, Ca and Mg. The 30% compost and 30% vermicompost treatments led to the maximal reduction of heavy metals in enhanced tobacco NBCu 10-8F3. The addition of compost and vermicompost further reduces the ability to digest the heavy metals in the leaves, and phytoremediation potential of enhanced tobacco NBCu 10-8F3.

Key words

heavy metals, micro and macroelements, organic meliorants, enhanced tobacco NBCu 10-8F3, phytoremediation


[1] Salt D. E., Smith R.D., Raskin I. (1998) Phytormediation. Annual Rev. Plant Physiol. Plant Mol. Biol., vol.49, pp.643 –668.
[2] Mench, M., Martin, E. (1991) Mobilization of cadmium and other metals from two soils by root exudates of Zea mays L., Nicotiana tabacum L., and Nicotiana rustica L., Plant and soil, vol.132, no.2,pp. 187-196.
[3] Mench, M.J., Didier, V.L., Loеffler, M., Gomez, A., Masson, P. (1994) A mimicked in-situ remediation study of metal contaminated soils with emphasis on cadmium and lead, J. Environ. Qual., vol. 23, pp.58-63.
[4] Guadagnini, M.. (2000) In vitro-Breeding for Metal-Accumulation in Two Tobacco (Nicotiana tabacum) Cultivars, Innagural-Dissertation No. 1288 derMathematisch Naturwissenschftlichen Fakult¨at der Universit¨at Freiburg in der Schweiz. pp. 109.
[5] Herzig, R., Guadagnini, M., Rehnert, A., Erismann, K.H. (2003) Phytoextraction efficiency of in vitro bred tobacco variants using a non-GMO approach. In: Vanek T, Schwitzgu´ebel JP, editors. Phytoremediation Inventory – COST Action 837 View. Prague (Czech Republic): UOCHB AVCR. p. 73.
[6] PHYTAC (2005) Development of systems to improve phytoremediation of metal contaminated soils through improved phtoaccumulation (QLRT-2001-00429 & 2001-02778 NAS). Final report 5th Framework Programme, Dec. 2005, p. 160–190.
[7] Clemente, R., Walker, D.J., Bernal, M.P. (2005) Uptake of heavy metals and As by Brassica Juncea grown in a contamination soil in Arnalcollar (Spain): The effect of soil amendments, Environmental Pollution, vol.136, pp.46 – 58.
[8] Walker, D. J., Clemente, R., Roig, A., Bernal, V. (2003) The effect of soil amendments on heavy metal bioavailability in two contaminated Mediterranean soils, Environ Pollution, vol.22, pp. 303 – 312.
[9] Hu, Z. (2004) Effect of bio-organic compound vermicompost on improvement of tobacco quality, Chinese Agricultural Science Bulletin, vol.20, no.3, pp. 157-158.
[10] Wang, S.S., Shi, Q.M., Li, W.Q., Niu, J.F., Li, C.J., Zhang, F.S. (2008), Nicotine concentration in leaves of fluecured tobacco plants as affected by removal of the shoot apex and lateral buds, J. Integr. Plant Biol., vol.50, pp. 958-964.
[11] Cao, P.Y., Lu, S.J., Zhang, W.S. (2004) Advances in soil organic matter contents and vermicomposts application in tobacco growing areas, Acta Tabacaria Sinica, vol.10, no.6, pp 40-42.
[12] Li, C.X. (2007) Effect of EM bio-organic vermicompost on tea garden, J. Tea Business, vol.29, no.2, pp. 65-66.
[13] ISO 11466 (1995) Soil quality. Extraction of trace elements soluble in aqua regia.
[14] ISO 14870 (2001) Soil Quality- Extraction of Trace Elements by Buffered DTPA Solution.
[15] del Piano, L., Abet, M., Sorrentino, Barbato, L., Sicignano, M., Cozzolino, E., Cuciniello, A. (2008) Uptake and distribution of lead in tobacco (Nicotiana tabacum L.), Journal of Applied Botany and Food Quality, vol.82, pp.21 – 25, 2008.
[16] Wagner, G.J.,Yergan, R (1986) Variation in Cd accumulation potential and tissue distribution of Cd in tobacco, Plant. Physiol., vol. 82, pp. 274-279.
[17] Fässler, E., Robinson, B.H., Gupta, S.K,, Schulin, R. (2010) Uptake and allocation of plant nutrients and Cd in maize, sunflower and tobacco growing on contaminated soil and the effect of soil conditioners under field conditions, Nutrient Cycling in Agroecosystems, vol.87(3), pp. 339–352.
[18] Anonуmous (1995) Encyclopedia of Analytical Science, Academic Press: London, San Diego New York, Boston, Tokyo, Toronto, vol.1, pp.418-419.
[19] Yeargan, R., Maiti, I.B., Nielsen, M.T., Hunt, A.G., Wagner, G.J. (1992) Tissue partitioning of cadmium in transgenic tobacco seedlings and field grown plants expressing the mouse metallothionein I gene, Transgenic Res., vol.1, pp.261-267.
[20] Tso, T. C. (1991) Production, physiology and biochemistry of tobacco plant. Beltsville, MD, Ideals.
[21] Lugon-Moulin, N., Zhang, M., Gadani, F., Rossi, L., Koller, D., Krauss, M., Wagner, G. I. (2004) Critical review of the science and options for reducing cadmium in tobacco (Nicotiana tabacum L.) and other plants, Adv. Agron.,vol. 83, pp.111-180.
[22] Golia, E.E., Dimirkou, A., Mitsios, I. K. (2007) Accumulation of metals on tobacco leaves (primings) grown in an agricultural area in relation to soil, Bull Environ Contam Toxicol., vol.79 (2), pp. 158-162.
[23] Mench, M.J., Didier, V.L., Loеffler, M., Gomez, A., Masson, P. (1994) A mimicked in-situ remediation study of metal contaminated soils with emphasis on cadmium and lead, J. Environ. Qual., vol. 23, pp.58-63.
[24] Sappin-Didier, V., Mench, M., Gomez, A., & Masson, P. (1997) Evaluation by single extractants of Cd immobilization following the addition of inorganic amendments in two metal contaminated soils, Comptesrendus de l.academie des sciences. Serie III. Sciences de la Vie, vol. 320, pp. 413-419.
[25] Adamu, C.A., Mulchi, C.L., Bell, P.F. (1989) Relationships between soil pH, clay, organic matter and CEC [cation exchange capacity] and heavy metal concentrations in soils and tobacco, Tob. Sci., vol. 33, pp. 96-100.
[26] Bell, P.F., Mulchi, C.Z., Chaney, R.Z. (1992) Microelement concentration in Maryland air-cured tobacco, Commun. Soil Sci. Plant Anal., vol.23, no.13-14, pp. 1617-1628.
[27] Kabata-Pendias, A., Pendias, H. (2001) Trace elements in soils and plants, 3rd ed. CRC, New York.
[28] Jones, J., Wolf, B., Mills, H.A. Plant Analysis Handbook. Micro-Macro Publishing Inc., 1991.
[29] Campbell, C. Reference Sufficiency Ranges Field Crops, Tobacco, Flue-cured, 2000.
[30] Wagner, G.I. (1994) Accumulation of cadmium in crop plants and its consequences to human health, Adv. Agronomy, vol.51, pp.173-212.
[31] Yancheva, D. (2002) Mineral composition of the oriental tobacco leaves depending on the nitrogen vermicompost rate”, In Proc. The Second Balkan Scientific Conference Quality and efficiency of the tobacco production, treatment and processing, Plovdiv, pp.162-166.


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