Violina Angelova – Agricultural University-Plovdiv, 12 Mendeleev street, Plovdiv, Bulgaria




3rd International Scientific Conference on Recent Advances in Information Technology, Tourism, Economics, Management and Agriculture – ITEMA 2019 – Bratislava, Slovakia, October 24, 2019, CONFERENCE PROCEEDINGS published by the Association of Economists and Managers of the Balkans, Belgrade; Printed by: SKRIPTA International, Belgrade, ISBN 978-86-80194-23-3, ISSN 2683-5991, DOI:




Comparative research has been conducted to allow us to determine the content of heavy metals
and chemical composition of Salvia officinalis L oils, as well as to identify the possibility of Salvia
officinalis L. growth on soils contaminated by heavy metals. The experimental plots were situated at
different distances of 0.5 km and 15 km, respectively, from the source of pollution the Non-Ferrous-Metal
Works (MFMW) near Plovdiv, Bulgaria. On reaching flowering stage the Salvia officinalis L plants
were gathered. The content of heavy metals in different parts of Salvia officinalis L. (roots, stems, leaves
and inflorescences) was determined by ICP. The essential oil of the Salvia officinalis L. was obtained
by steam distillation in laboratory conditions which was analyzed for heavy metals and its chemical
composition was determined.
Salvia officinalis L. is a plant which is tolerant to heavy metals and can be grown on contaminated soils.
Favorable is also the fact that heavy metals do not influence the development of the Salvia officinalis
L. as well as on the quality and quantity of the essential oil. Twenty-nine components were identified
in the oil. The main compounds of essential oi were as follows: α-thujone (15.927-18.912), camphor
(16.839-17.826), trans-thujone (5.379-11.575), 1,8-cineole (6.891-7.625), camphene (6.024-6.514), α-humulene
(5.395-6.098), borneol (4.591-5.394), (e)-β-caryophyllene (3.868-4.576), limonene (3.458-4.612),
bornyl acetate (1.621-3.194), viridiflorene (2.449-5.633), β-pinene (2.174-2.223), allo-aromadendrene
(2.034-4.777). The compounds in the essential oil that decreased as a result of heavy metals pollution
are limonene, bornyl acetate and allo-aromadendrene, while the α-thujone, trans-thujone, camphor
and viridiflorene significantly increased. Observed increase of the levels of α-thujone, trans-thujone,
and camphor level in the leaves of sage grown on heavy metals polluted soil indicated an improvement
of the essential oil quality. The essential oil of Salvia officinalis L. can be a valuable product for the
farmers from the polluted regions.



Heavy Metals, Contaminated Soils, Essential Oil Composition, Salvia Officinalis


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